14/09/17 20:13:40.35
【小保方らが2012年7月に Science 誌に投稿したときの査読結果全文公開】
"Truly extraordinary," "simply not credible," "suspiciously sharp:"
A STAP stem cell peer review report revealed
URLﾘﾝｸ(retractionwatch.com)
Reviewer 1 は捏造であることをほぼ完全に看破していた:
> I suspect that the results are artifacts derived from the following processes:
> (1) the tendency of cells with GFP reporters to go green as they are dying.
> (2) the ease of cross contamination of cell lines kept in the same lab.
> I believe that the green transformation is indeed due to stress as reporters are
> often upregulated in stressed or dying cells. But the cells that go green may not
> be the ones in the later green colonies. I think these are more likely to be ES cells
> acquired by cross contamination and selected for growth by the B27-LlF medium.
> In Fig.2B and the other RT-PCR studies, it is not stated whether the Y-axis is linear
> or logarithmic. If it is linear, which seems more likely, then I am very surprised that
> all of the pluripotency genes measured in the ESC control have virtually the same RNA
> abundance, which exceeds that of GAPDH.
> The DNA analysis of the chimeric mice is the only piece of data that does not fit
> with the contamination theory. But the DNA fragments in the chimeras don' t look the same
> as those in the lymphocytes. This assay is not properly explained. If it is just an
> agarose gel then the small bands could be anything. Moreover this figure has been
> reconstructed. It is normal practice to insert thin white lines between lanes taken
> from different gels (lanes 3 and 6 are spliced in). Also I find the leading edge of
> the GL band suspiciously sharp in #2-#5.