14/07/02 20:33:20.73
URLﾘﾝｸ(www.nature.com)
(1) Figure 1a and b in the Letter both show embryos generated from STAP cells, not a comparison of ES- and STAP-derived chimaeric embryos, as indicated in the legend.
(2) Extended Data Fig. 7d in the Article and Extended Data Fig. 1a in the Letter are different images of the same embryo and not, as indicated in the legends, a diploid chimaera embryo and tetraploid chimaera embryo.
(3) There is an erroneous description in Fig. 1a in the Letter. The right panel of Fig. 1a is not a ‘long exposure’ image at the camera level but a digitally enhanced one.
(4) In Fig. 4b of the Letter, STAP cell and ES cell are wrongly labelled in a reverse manner.
(5) In the Article, one group of STAP stem cells (STAP-SCs) was reported as being derived from STAP cells induced from spleens of F1 hybrids from
the cross of mouse lines carrying identical cag-gfp insertions in chromosome 18 in the background of 129/Sv and B6,
respectively, and that they were maintained in the Wakayama laboratory.
However, further analysis of the eight STAP-SC lines indicates that, while sharing the same 129×B6 F1 genetic background,
they have a different GFP insertion site. Furthermore, while the mice used for STAP cell induction are homozygous for the GFP transgene, the STAP-SCs are heterozygous.
The GFP transgene insertion site matches that of the mice and ES cells kept in the Wakayama laboratory.
Thus, there are inexplicable discrepancies in genetic background and transgene insertion sites between the donor mice and the reported STAP-SCs.
意訳
(1) なんと！どっちのキメラもSTAP細胞由来です！
(2) 説明文間違ってただけです！
(3) 長時間露出じゃなくて画像処理でしたテヘ
(4) ラベリングミスでぁ～す
(5) 若山が悪い若山が悪い若山が悪い若山が悪い若山が悪い若山が悪い若山が悪い若山が悪い若山が悪い若山が悪い若山が悪い若山が悪い若山が悪い若山が悪い若山が悪い