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2.3 Results
2.3.1 Sphere forming from isolated cells
Trituration and optimistic pressure produced spheres. Whereas, cell sorter did not
produced any spheres. Trituration produced most number of spheres. Therefore, we
utilized trituration as the isolation method of small cells.
2.3.2 Effect of trituration
After the trituration, the population of small cells was increased comparing to
native bone marrow cells (Fig. 3A and B). However not all of cells over 8 micro
meters in diameter disappeared. Triturated cells formed spheres during culture.
Interestingly, spheres were consisted of only small cells (Fig. 3C). Thus, it was
demonstrated that trituration enabled to culture and grow only small cells.