14/03/06 01:53:17.46
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IMPORTANT
(i) The buffering action of HBSS is weak, so carry-over of the solution may affect pH.
Please adjust pH to 5.7 in cell suspension by the following method.
First, suspend the cell pellet with 494 μl of HBSS pre-chilled at 4°C,
then add 6 μl of diluted HCl (10 μl of 35% HCl in 590 μl of HBSS) to adjust to a final pH of 5.7.
Please confirm the final pH in a pilot experiment, and optimize the volume of HCl added, as necessary.
Alternatively, suspend the cell pellet in HBSS-pH 5.4 pre-chilled at 4°C.
(ii) The HBSS we used is Ca2+/Mg2+ free (Gibco 14170-112).
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