14/03/06 00:23:25.17
わざわざ
Different lots of FBS may results in significant differences in the
behavior of cultured cells.
とFBSのロットが重要だと書きながら，どこのをつかったのか一切書いてない．
(ii) Alternatively, red blood cells may be removed by suspension of the cell
pellet in 1.8 ml of H2O (Sigma W3500). After 30 seconds, add 0.2 ml of 10×
PBS (Gibco 70011-044), followed by 3 ml of 1× PBS (Gibco 10010-023), and
strain the cell suspension through a cell strainer.
水やPBSは型番まで指定されるのにｗｗｗ
再現できないのではFBSが悪いせいで押し通す気か