14/02/27 21:02:18.51
コピペ について みなさん OAされたNature Article 確認されていますでしょうか?
To the cell pellet in 3 ml of PBS, 7 ml of a pre-warmed hypotonic 0.0375M KC1
solution was added. Cells were incubated for 20 min at 37 uC. Cells were centrifuged
for 5 min at 1,200 r.p.m. and the pellet was re-suspended in 3–5 ml of 0.0375M
KC1 solution. The cells were fixed with methanol/acetic acid (3:1; vol/vol) by
gently pipetting. Fixation was performed four times before spreading the cells
on glass slides. For the FISH procedure, mouse chromosome-specific painting
probes were combinatorially labelled using seven different fluorochromes and
hybridized as previously described41. (41は上付き)
そのページの下部に
41. Jentsch, I., Geigl, J., Klein, C. A. & Speicher, M. R. Seven-fluorochrome mouse
M-FISH for high-resolution analysis of interchromosomal rearrangements.
Cytogenet. Genome Res. 103, 84-88 (2003).
とあります。41を入れる場所がもっと上だったら明確ですが、この段落の該当箇所まで全てに対して41のRefをつけたかったものと想像されます。