14/02/24 17:47:15.68
海外の反応
図1aは、実験モデルの説明。
図1bは、懸濁液中の細胞で、pH処理後に特異的にOct4-GFPが現れる図だ。問題点
はこの緑が自家発光か、それともほんと発現して発光しているのかと言う点だ。
また、この3つのパネルをほんとに同じ操作をして、同じように写真をとってるか
どうか。
図1cは7日目のOct4-GFPの発現をFACSで解析した結果だ。上側の2つのパネルがpH処理
したものだけども7日目には、驚いたことにほぼ100% Oct4-GFPが発現してるよね。
図1dで、7日目の、このpH処理した細胞のOct4-GFPの割合が50%となってるけど
完全に図1cと矛盾してるよね。
■Figure 1a is their experimental model.
■Figure 1b reports that an Oct4-GFP reporter comes on specifically
after low-pH treatment in spheres of cells in suspension. Concerns
have been raised that the green signal may have been enhanced and/or
could be autofluorescence, and the 3 panels may not have been
captured and/or manipulated in an equivalent manner to each other.
■Figure 1c is FACS analysis of the Oct4-GFP reporter turning on
at day 7. The upper 2 panels are low-pH treated cells. By day 7
this data would suggest that basically 100% of cells became GFP+,
a stunning reported finding.
■Figure 1d is a quantification of viable cells by relative GFP
status reporting a conversion to a GFP+ state in about 50% of cells
by d7. This would seem to conflict with Figure 1c.