14/02/24 12:31:02.16
フランクフルト 月曜日に取り出して解析するとの事
dr.p
on February 22, 2014 at 12:36 am said:
Hi Anne,
I am not hiding anything, I just don´t want to put up a blot and get slaughtered because it does´t meet the requirements of many other scientist reading this blog.
The only success I have had with STAP so far is Oct4 expression from unsorted adult rat spleenocytes at 7 days. These cells do not contain any GFP transgene.
This is being repeated and if it works I will share it here.
As pertains to your GFP questions: I have no idea if the STAP worked on the spleenocytes from the Oct4GFP mouse.
I will take the cells out of culture on monday, analyse them and report back.
I have no intentions of making teratomas or studying TCR rearrangements.
I did try the STAP protocol on MEFs, but I wash´t´happy with the experiment and I am repeating it.