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Nontypeable Haemophilus influenzae and Streptococcus pneumoniae bind respiratory syncytial virus glycoprotein
Vasanthi Avadhanula,1,2 Yan Wang,1 Allen Portner1,2 and Elisabeth Adderson1,2,3
Journal of Medical Microbiology (2007), 56, 1133?1137
URLﾘﾝｸ(jmm.sgmjournals.org)
サマリー
Respiratory syncytial virus (RSV) infection is associated with secondary bacterial infections
caused by nontypeable Haemophilus influenzae (NTHi) and Streptococcus pneumoniae.
The pathogenesis of these complications is not completely understood; however,
viral infection of respiratory epithelial cells promotes colonization by these bacteria. In the present study,
RSV virions associated with NTHi and pneumococci in an inoculum-dependent manner in a fluid-phase binding assay.
Adherence of NTHi and S. pneumoniae to epithelial cells transiently expressing RSV G glycoprotein
was 2- and 2.2-fold higher, respectively, than adhesion to cells transfected with the vector alone (P ,0.01).
Furthermore, 4.6- and 6.2-fold larger numbers of NTHi and pneumococci bound to cells expressing
a membrane-bound full-length RSV G protein than to cells expressing a truncated non-membrane-bound protein (P !0.005).
Pre-incubating cells expressing membrane-bound G protein with blocking anti-RSV G antibodies reduced bacterial
adherence by 78?84% (P !0.005).
These studies demonstrate that RSV G protein is a receptor for both NTHi and S. pneumoniae.
Strategies to prevent this interaction may reduce the incidence of secondary bacterial complications of RSV infection.