19/04/17 14:30:09.79 7wp+2qRW0.net
長文ですが、こちらの翻訳をお願いします。医学用語なので難しいです。
Microsomal cytochrome P450 enzymes (CYPs) are membrane-attached enzymes
that play indispensable roles in biotransformations of numerous endogenous and
exogenous compounds. Although recent progress in experiments and simulations
has allowed many important features of CYP-membrane interactions to be deciphered,
many other aspects remain underexplored. Using microsecond-long molecular dynamics
simulations, we analyzed interaction of CYP3A4 with bilayers composed of lipids differing
in their polar head groups, i.e., phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine,
and phosphatidylglycerol. In the negatively charged lipids, CYP3A4 was immersed more deeply
and was more inclined toward the membrane because of favorable electrostatic and hydrogen
bonding interactions between the CYP catalytic domain and lipid polar head groups.
We showed that electrostatics significantly contributes to positioning and orientation of CYP
on the membrane and might contribute to the experimentally observed preferences of individual
CYP isoforms to distribute in (dis)ordered membrane microdomains.