14/06/15 01:39:33.37
>>148
>Knoepfler BlogではOct4-GFPの話しかしてない
違うな。
(Knoepfler Blog参照)
URLﾘﾝｸ(www.ipscell.com)
>I established STAP-SC several times from STAP.
>It is unlikely that contamination would always have happened.
>In addition, we established STAP-SC from 129B6GFP mice.
>At that time, we did not have this strain ES cell.
>
>When I succeeded to establish STAP-SC,
>the original STAP cells expressed Oct4-GFP very much.
>In this condition, the establishment is much easier than
>ES cell establishment from blastocyst.
「129B6GFP mice」と「the original STAP cells expressed Oct4-GFP very much」
は別物。
キメラ作成用のGFPは常光性のGFP。Oct4-GFPだと妊娠後期になると
緑に蛍光するのが生殖器だけになるから。